[Abstract] Objective To explore the influencing factors of Barrett’s esophagus after endoscopic submucosal dissection (ESD) in patients with early esophageal cancer.Methods 110 patients with esophageal cancer who received ESD therapy in our hospital from January 2022 to March 2023 were divided into two groups according to whether they had Barrett’s esophagus (n=20) and no Barrett’s esophagus (n=90).The general clinical data,esophageal cancer related characteristics and ESD related treatment characteristics of the two groups were compared.Multivariate regression analysis were used to screen the risk factors of Barrett’s esophagus in patients with esophageal cancer after ESD.Results The proportion of male,former drinking,former smoking,prevalence of peptic ulcer,multiple tumors,maximum diameter of tumors,the rate of postoperative lymph node metastasis,postoperative distant metastasis and the proportion of muscularis propria injury in patients with Barrett’s esophagus were significantly higher( P<0.05).Multivariatelogistic regression analysis showed that alcohol drinking,smoking,peptic ulcer,postoperative lymph node metastasis,postoperative distant metastasis and injury of muscularis propria were the risk factors for Barrett’s esophagus ( P<0.05).Conclusion Patients with a history of drinking,smoking,peptic ulcer,postoperative lymph node metastasis,postoperative distant metastasis and injury of muscularis propria are more likely to develop Barrett’s esophagus after ESD.

[Abstract]Objective To analyze the expression levels of neuron specific enolase (NSE),stromal cell derived factor-1 (SDF-1) and decoy receptor 3 (DcR3) in serum of patients with non-small cell lung cancer (NSCLC) and their prognostic value.Methods From March 2019 to March 2020,132 NSCLC patients admitted to Yixing Hospital of Traditional Chinese Medicine and the People’s Hospital of Subei were collected as the study subjects (NSCLC group),another 132 healthy examinees who came to our hospital were as the control group.enzyme linked immunosorbent assay (ELISA) was applied to detect the expression levels of NSE,SDF-1,and DcR3 in serum;multivariatenLogisticn regression was applied to analyze the influencing factors of poor prognosis in NSCLC patients; and receiver operating characteristic (ROC) curve was applied to analyze the value of serum NSE,SDF-1,and DcR3 expression levels in evaluating the prognosis of NSCLC patients.ResultsThe expression levels of NSE,SDF-1,and DcR3 in the serum of the NSCLC group were obviously higher than those of the control group,with statistical significance ( P<0.05);the expression levels of NSE,SDF-1,and DcR3 in the serum of NSCLC patients with a tumor diameter of ≥ 3 cm,low tumor differentiation,TNM staging of stage Ⅲ-Ⅳ,and lymph node metastasis were obviously higher than those of NSCLC patients with a tumor diameter of<3 cm,high/medium tumor differentiation,TNM staging of stage Ⅰ-Ⅱ,and no lymph node metastasis ( P<0.05);compared with the good prognosis group,the expression levels of NSE,SDF-1,and DcR3 in the serum of NSCLC patients in poor prognosis groupwere obviously increased,and the proportion of lymph node metastasis was obviously increased( P<0.05);the results of multivariatenLogisticn regression analysis showed that NSE,SDF-1,DcR3,and lymph node metastasis were the influencing factors for poor prognosis in NSCLC patients ( P<0.05);the results of receiver operating characteristic analysis showed that the area under the curve (AUC) of serum NSE,SDF-1 and DcR3 levels in evaluating prognosis of NSCLC patients was 0.852,which was more valuable than the single index of NSE,SDF-1 and DcR3.Conclusion The expression levels of NSE,SDF-1,and DcR3 in the serum of NSCLC patients are obviously increased,and the combination of the three can be used as a prognostic indicator for NSCLC patients.

[Abstract] Objective To investigate the expression of X-linked inhibitor of apoptosis associated factor 1 (XAF1) and phosphoenolpyruvate carboxykinase 1 (PCK1) in Luminal type B breast cancer and their relationship with the prognosis of patients.Methods From January 2016 to January 2018, 95 patients with Luminal B breast cancer underwent surgical treatment. Cancer tissues and adjacent tissues > 5 cm from the cancer tissues were collected during the operation.The positive expressions of XAF1 and PCK1 proteins were detected by immunohistochemistry.The relationship between XAF1 and PCK1 protein expression and clinicopathological features of breast cancer was analyzed, and the survival curves of breast cancer patients with different XAF1 and PCK1 protein expression were plotted by KaplanMeier method,the survival rate was compared by Log-rankn test. Multi-factor Cox-regression analysis was used to analyze the prognostic factors of breast cancer.Results GEPIA database analysis showed that the expression levels of XAF1 and PCK1 mRNA in breast cancer tissues were lower than those in normal tissues ( P<0.05).The positive expression rates of XAF1 and PCK1 proteins in cancer tissue were lower than adjacent tissues ( P<0.05).The positive expression of XAF1 in breast cancer tissue was related to clinical stage,lymph node metastasis and histological grade,Ki-67 expression status,while the positive expression of PCK1 was related to clinical stage,lymph node metastasis and Ki-67 expression status ( P<0.05).The 5-year overall survival rate of XAF1 positive expression patients was higher than that of negative expression patients (Log-rank χ²=5.101， P=0.024),and the 5-year overall survival rate of PCK1 positive expression patients was higher than that of negative expression patients (Log-rank ^χ2=6.515, P=0.011).Clinical stage Ⅲ,negative expression of XAF1,and negative expression of PCK1 were risk factors for poor prognosis ( P<0.05).Conclusion The positive expression rates of XAF1 and PCK1 in Luminal B type breast cancer tissue are reduced,which is related to some clinicopathological characteristics and 5-year survival rate after surgery.The 5-year survival rate was lower in patients with negative XAFI and PCKI expression.

[Abstract] Objective To summarize the operation techniques and experience of arthroscopic “piecrustiong” release for patellofemoral joint hypertension.Methods From August 2021 to April 2023,67 patients (75 knees) suffered from isolated patellofemoral compression syndrome.The lateral retinacular of knees were arthroscopic released by pie crusting technique.All knees were assessed by AKS and Kujala scores.Results The follow-up period ranged from 3 to 18 months.All patients had good wound healing without infection, joint hematosis, fluid accumulation, and limited movement.AKS score of knee joint was (44.88±5.49)points before surgery and (69.57±4.64)points 3 months after surgery. Kujala score was (47.08±6.28)points before surgery and (68.75±4.47)points 3 months after surgery. The Vas score was (2.81±0.67)points before surgery and (0.85±0.65）points 3 months after surgery.The AKS, Kujala and Vas scores of knee joint after treatment were higher than those before treatment, and the differences were statistically significant ( P < 0.05).Conclusion The treatment of arthroscopic “pie-crusting” technique can improve the patellar joint matching degree, relieve pain and improve quality of life.

[Abstract] Objective Exploring the efficacy and prognostic analysis of open reduction and internal fixation combined with ligament repair in the treatment of ankle joint fractures with anterior tibiofibular ligament rupture.Methods Ninety-two patients with ankle fracture combined with lower tibiofibular anterior ligament rupture admitted to our hospital from January 2020 to August 2022 were selected to implement the prospective study,and they were divided into 46 cases in the study group and 46 cases in the control group according to the different treatment modalities.The control group only received open reduction and internal fixation surgery,while the research group received open reduction and internal fixation surgery combined with ligament repair treatment.The differences in surgical indicators and recovery,pain level (VAS score),ankle joint function recovery (AOFAS score,ankle ROM),bone metabolism indicators,postoperative complications,and long-term prognosis between the two groups were compared.Results Compared with the control group,the study group had longer operation time,shorter hospitalization time,and shorter fracture healing time ( P<0.05).Repeated measures showed that there were differences in the comparison of timepoint effects,between-group effects,and interaction effects of VAS scores ( P<0.05),and VAS scores were lower in both groups at 1 month and 3 months postoperatively compared with preoperatively,and were better in the study group than in the control group ( P<0.05).Compared with preoperative,AOFAS score and ankle ROM were increased in both groups at 6 months postoperatively,and the study group was better than the control group ( P<0.05).Compared with preoperative,collagen type Ⅰ N-terminal peptide (PINP),osteocalcin (BGP),bone alkaline phosphatase (BALP) were elevated in both groups at 6 months postoperatively,and the level of β-collagen degradation product (β-CTX) β-CTX was reduced in the former ( P<0.05);and the study group was better than the control group ( P<0.05).Compared with the control group,there was no significant difference in the comparison of postoperative complication status ( P>0.05),but the study group had a better long-term prognosis ( P<0.05).Conclusion The combination of open reduction and internal fixation surgery with ligament repair for the treatment of ankle fracture combined with anterior tibiofibular ligament rupture has good clinical efficacy,can effectively reduce pain,improve ankle function,regulate bone metabolism level,promote fracture healing,and has good safety and long-term prognosis.