Abstract: Objective To explore the relationship between long-chain non-coding RNA MIR205HG (LncRNA MIR205HG) and microRNA-299-3p (miR-299-3p) and its molecular mechanism affecting proliferation and apoptosis of lung cancer cells. Methods  The expressions of MIR205HG and miR-299-3p in lung cancer tissue and cell lines were detected.The H1299 cells were grouped into:si-MIR205HG group,si-NC group,miR-299-3p group,miR-NC group,si-MIR205HG+anti-miR-299-3p group,si-MIR205HG+anti-miR-NC group.The interaction of MIR205HG with miR-299-3p was detected.Proliferation, apoptosis and protein expression were detected. Results The expression level of MIR205HG in lung cancer tissues and cell lines was increased (P<0.05),and the expression level of miR-299-3p was decreased (P<0.05).MIR205HG can specifically bind to miR-299-3p and regulate the expression and activity of miR-299-3p.Interference with MIR205HG expression or miR-299-3p overexpression reduced lung cancer cell proliferation and promoted apoptosis,and the expressions of CyclinD1 and p21 decreased,while the expressions of Bcl-2 and Bax increased (P<0.05).Inhibition of miR-299-3p reversed the effect of MIR205HG expression on proliferation and apoptosis of lung cancer cells. Conclusion Interfering with the expression of MIR205HG could inhibit lung cancer cell proliferation and induce cell apoptosis,and its mechanism of action was related to the negative regulation of miR-299-3p.

Key words: lung cancer, LncRNA MIR205HG, miR-299-3p, proliferation, apoptosis