Abstract: Objective To count and analyze the composition of pathogenic bacteria inabdominal pus and drug sensitivity test results of patients with acute appendicitis.Methods From January 1, 2020 to January 1, 2021, 280 patients with acute appendicitis received laparoscopic appendectomy. Intraoperatively, abdominal fluid was taken from the patients, and bacterial culture and drug sensitivity tests were performed.Bacteria were identified by VTEK-MS mass spectrometer; drug susceptibility tests were performed by VITEK2-Compact automatic microbiological analyzer or disk diffusion.Result In terms of the composition of pathogenic bacteria,Escherichia coli was the main pathogenic bacteria,accounting for 59.7%,followed by pseudomonas aeruginosa (11.2%) and klebsiella pneumoniae (6.6%).In drug susceptibility test,the five antibiotics with the highest sensitivity to Escherichia coli  were:carbapenems(100%),amikacin(98.3%),piperacillin/tazobactam(97.4%),cefoxitin(95.7%),amoxicillin/clavulanate potassium (85.5%).Conclusion The sensitivity rates of piperacillin/tazobactam,cefoxitin,amoxicillin/clavulanate potassium,amikacin and carbapenems to Escherichia coli in patients with acute appendicitis are all above 70% ,which can be preferred according to clinical situation.

Key words: acufte appendicitis, abdominal pus, germiculture, drug sensitive test