Abstract: Objective To investigate the survival rate and filling effect of autologous fat granules treated at 4 ℃.
Methods From January 2018 to April 2019,100 patients who were selected for frontotemporal autologous fat filling surgery were randomly divided into two groups:Group Ⅰ(normal temperature group,n=50):The extracted autologous fat was washed,purified with normal temperature saline and stored at normal temperature.Group Ⅱ(cryogenic group,n=50):The extracted autologous fat was washed,purified and cryopreserved with 4 ℃ cryogenic saline,and then the purified autologous fat was filled in frontotemporal region.Patients were interviewed and the filling effect was evaluated at 1,3 and 6 months after surgery.
Results After autologous fat filling,the total satisfaction rate of the surgeon to the two group patients at different times gradually decreased(100%,94%,86%;100%,98%,94%),but there was no significant difference between the two groups(P＜0.05).The number of dissatisfied patients in group Ⅰ at six months after surgery(32%) was significantly more than that at one month after surgery(6%),and it was also significantly more than that in group Ⅱ at six months after surgery(10%)(P＜0.05);The time of redness and swelling in group Ⅰ[(68.4±10.2)h] was significantly longer than that in group Ⅱ[(52.6±8.9)h].The number of patients in group Ⅰ(28%) who required secondary filling after autologous fat filling was also significantly higher than that in group Ⅱ(6%)(P＜0.05).However,there was no significant difference in the number of patients with postoperative complications between the two groups(P>0.05).
Conclusion Autologous fat which was washed,purified and stored at 4 ℃ low temperature is more tolerant to anoxia injury and improve filling effect.

Key words: low temperature, autologous fat, temporal forehead, transplant