Abstract: Objective：To observe the clinical value of intraoperative methylene blue(MB) nearinfrared fluorescence imaging and mapping in the sentinellymph node biopsy(SLNB) of early stage breast cancer.Methods：The clinical datas of 79 patients of early stage breast cancer(cT12N0M0)who were admitted from January 2018 to December 2019 in the department of thyroid & breast of Hubei Maternal and Children’s Hospital were analyzed retrospectively. These cases were divided into two groups according to admission orders:control group(A) and observation group(B).Group A (39 cases) were applied by intraoperative MB mapping of SLNs.Group B(40 cases) were applied by intraoperative MB nearinfrared fluorescence imaging and mapping of SLNs.To compare the biopsy results and surgical indicators of the two groups.Results：The two groups of SLNB success rates were 100%.There were significant differences in the aspects of the numbers lymph nodes detected and the operation time of the two groups (P<0.05).The mean operation time of the observation group(B) was less than the control group(A).The mean numbers of the lymph nodes detected in observation group(B) were less than the control group(A) .There were no significant differences in the aspects of the intraoperative blood loss, surgical incision complications (such as incision infection/dehiscence, incision hydrops) of the two groups(P>0.05).Conclusion：Compared with simple MB mapping, the application by intraoperative MB nearinfrared fluorescence imaging and mapping was more intuitive, simple, efficient for SLNB of the early stag breast cancer and there was high clinical value for it. 

Key words: methylene, 〓florescence, 〓breast cancer, 〓sentinel lymph node, 〓biopsy