Abstract: Objective To investigate the primary mechanism of neurological dysfunction after blast injury in rats.
Methods There were 32 healthy male SD rats were randomly divided into the control group and the blast injury group.The blast injury model was made with TNT explosion.The neurobehavioral score of the two groups were detected by Garcia scoring system.Immunofluorescence staining was used to determine the apoptosis of hippocampal neurons,and immunohistochemistry was used to examine the difference in the expression of ZO-1 protein in two groups.
Results The neurobehavioral scores of rats in blast injury group were significantly lower than those in control group(P<0.05).The closer to the explosion center was,the lower the neurobehavioral score was.TUNEL test showed that the apoptosis rate of nerve cells in the blast injury group was significantly higher than that in the control group,and immunohistochemical results showed that the positive expression rate of ZO-1 protein in the blast injury group was significantly lower than that in the control group(P<0.05).
Conclusion Blast injury has an obvious impairment effect on neurological function in rats,which may be caused by BBB damage and neuronal apoptosis.

Key words: blast injury, neurological dysfunction； blood-brain barrier