Abstract: Objective：To investigate the effect of highly selective minimally invasive treatment of varicocele and its effect on serum indexes.Methods：To screening 120 cases of the patients with varicocele as research subjects，which were divided into study group with 60 cases and control group with 60 cases，patients in control group were given laparoscopic cluster spermatic vein ligation and patients in study group were given laparoscopic highselectivity spermatic vein ligation，then the two groups were compared in terms of clinical efficacy and so on.Results：The clinical efficiency in study group（95.0%） was higher than the control group（80.0%）（P<0.05）；There were no significance in immune indexes（P＞0.05）.The immune indexes of IgG，IgA and IgM in study group were （16.8±2.2）g/L，（2128.2±583.0）mg/L，（2518.4±412.2）mg/L，respectively；while in control group were （11.5±1.8）g/L，（1643.9±421.5）mg/L，（1711.7±336.8）mg/L，respectively.The difference between the two groups was statistically significant（P＜0.05）.There was no difference in serum reproductive hormone T，LH and FSH between the two groups（P＞0.05）.The reproductive hormone indexes of T，LH and FSH after surgery in study group were（1.90±0.27 ）ng/ml，（2.42±0.50） mIU/m，（1.45±0.12） mIU/ml，respectively；while in control group were（1.78±0.24 ）ng/ml，（2.84±0.51） mIU/ml，（1.67±0.13） mIU/ml，respectively（P＜0.05）.There was no significant difference in sperm concentration，sperm motility and sperm malformation rate between the two groups（P＞0.05）.Postoperative sperm concentration in the study group was（53.20±7.46）×106/ml，the sperm activity was （72.62±9.52）%，the rate of sperm malformation was （14.35±5.14）%，while in control group were（44.58±7.54）×106/ml，（62.86±8.53）%，（19.66±6.29）%，respectively（P＜0.05）.Conclusion：The laparoscopic highselectivity spermatic vein ligation in the treatment of the patients with varicocele is significant，it is worthy of popular〖LM〗ization and application.

Key words: laparoscopic highselectivity spermatic vein ligation, varicocele, immune function, reproductive hormone, semen quality