Abstract: Objective To investigate the impacts of butorphanol on the proliferation,apoptosis and migration of esophageal cancer cells and its regulation on CCL2-CCR2 axis.Methods Human esophageal cancer KYSE30 cells were treated with 0~400ng/ml of butorphanol gradient concentration,and the cell proliferation was detected by MTT method;KYSE30 cells were grouped into control group,butorphanol L group,butorphanol M group,butorphanol H group and butorphanol H+CCL2 group,EdU method was applied to detect cell proliferation;cell apoptosis was detected by Hoechst method;cell migration was detected by cell scratch test;Transwell method was applied to detect cell invasion;Western blot was applied to verify the expression of CC chemokine ligand 2 (CCL2) and CC chemokine receptor 2 (CCR2).Results Butorphanol at the concentrations of 50ng/ml,100ng/ml,200ng/ml and 400ng/ml obviously inhibited the proliferation of KYSE30 cells;compared with the control group,the proliferation,migration and invasion of KYSE30 cells and the expression of CCL2 and CCR2 proteins in butorphanol L,M,H groups and butorphanol H+CCL2 groups were obviously decreased (P<0.05);compared with butorphanol H group,the cell proliferation,migration,invasion abilities and the expression levels of CCL2 and CCR2 proteins in butorphanol H+CCL2 group were obviously increased (P<0.05).Conclusion Butorphanol can obviously inhibit the proliferation,migration and invasion of esophageal cancer KYSE30 cells and promote cell apoptosis,which may be related to its inhibition of CCL2-CCR2 axis.

Key words: butorphanol, esophageal cancer cells, CC chemokine ligand 2-CC chemokine receptor 2 axis, proliferation, apoptosis, migration, invasion