Abstract: Objective To explore whether lncRNA SNHG16 could regulate the proliferation,migration,invasion and apoptosis of Wilms’ tumor WIT49 cells by targeting miR-22-3p.
Methods qRT-PCR was used to detect the expression levels of SNHG16 and miR-22-3p in Wilms’ tumor tissues and adjacent tissues.Human Wilms’ tumor WIT49 cells were cultured in vitro,and si-NC,si-SNHG16,miR-NC,miR-22-3p mmics,si-SNHG16 and anti-miR-NC,si-SNHG16 and anti-miR-22- 3p was transfected into WIT49 cells.The qRT-PCR method was used to detect the expression levels of SNHG16 and miR-22-3p in the cells.The CCK-8 method was used to detect cell proliferation.Transwell cell experiment was used to detect cell migration and invasion.Flow cytometry was used to detect the apoptosis rate.The dual luciferase reporter experiment detects the targeting relationship of SNHG16 and miR-22-3p.Western blot method was used to detect the protein expression of CyclinD1,MMP-2,MMP-9,Bcl-2,p21,Bax.〖WT5”HZ〗Results Compared with adjacent tissues,the expression level of SNHG16 in Wilms’ tumor tissue was significantly increased(P＜0.05),and the expression level of miR-22-3p was significantly decreased(P＜0.05).Transfection of si-SNHG16 or miR-22-3p mimics could significantly reduce the OD value and the protein levels of CyclinD1,MMP-2,MMP-9 and Bcl-2(P＜0.05),and reduce the number of migration and invasion cells(P＜ 0.05),increase the apoptosis rate and the levels of p21 and Bax protein(P＜0.05).The dual luciferase report experiment confirmed that SNHG16 could target miR-22-3p.Down-regulating the expression of miR-22-3p could significantly reverse the effect of inhibiting the expression of SNHG16 on the proliferation,migration,invasion and apoptosis of Wilms tumor WIT49 cells.
Conclusion Inhibition of SNHG16 expression could reduce the proliferation,migration and invasion ability of Wilms’ tumor WIT49 cells by up-regulating the expression of miR-22-3p,and induce cell apoptosis.

Key words: lncRNA SNHG16, miR-22-3p, Wilms’ tumor, proliferation, migration, invasion, apoptosis