Abstract: Objective：To explore the diagnostic values of long noncoding RNA (lncRNA) ANRIL，matrix metalloproteinase2 (MMP2) and serum amyloid protein A (SAA) for esophageal cancer，and the relationship between the three and the clinicopathological features of esophageal cancer.Methods：80 cases of esophageal cancer diagnosed in our hospital from January 2016 to December 2017 were collected，and 100 healthy people were recruited in our hospital during the same period.The levels of lncRNA ANRIL in the peripheral blood of subjects were detected by realtime quantitative PCR (RTPCR).Enzymelinked immunosorbent assay (ELISA) and enzyme methods were used to detect MMP2 and SAA levels，respectively.Results：Serum lncRNA ANRIL，MMP2 and SAA levels in patients with esophageal cancer were significantly higher than those in healthy subjects，and the difference were statistically significant (P<0.05).In addition，the expressions of lncRNA ANRIL，MMP2 and SAA in serum of patients with esophageal cancer were significantly correlated with TNM stage，histology classification and differentiation degree of tumor(P<0.05)，and the levels of lncRNA ANRIL，MMP2 and SAA in advanced esophageal cancer，esophageal adenosquamous carcinoma and poorly differentiated esophageal cancer patients were increased most significantly.When combined with lncRNA ANRIL，MMP2 and SAA，the area under the curve (AUC) to distinguish between esophageal cancer and healthy subjects was 0.927（95% CI：0.900～0.954，P<0.05），the sensitivity was 80.1%，specificity was 90.4%.Multivariate analysis showed that serum lncRNA ANRIL，MMP2 and SAA levels were independent risk factors for esophageal cancer.Conclusion：lncRNA ANRIL，MMP2 and SAA have diagnostic values for esophageal cancer.In addition，lncRNA ANRIL，MMP2 and SAA can also serve as reference index for predicting the risk of esophageal cancer.

Key words: esophageal cancer, long noncoding RNA;matrix metalloproteinase-2;serum amyloid protein A