Abstract: [Abstract]〓Objective〖WTBZ〗〓To study the expression and clinical significance of p53，Ki67，Ecadherin and CD34 in gallbladder carcinoma.〖WTHZ〗Methods〖WTBZ〗〓We collected a total of 120 cases of cholecystectomy，which were divided into 3 groups：40 cases of gallbladder carcinoma（gallbladder cancer group），40 cases of paracancerous tissue（paracancer group），and 40 cases of normal gallbladder tissue（normal group）.Immunohistochemistry was used to detect the expression of p53，Ki67，Ecadherin and CD34 in the tissues.〖WTHZ〗Results〖WTBZ〗〓The positive expression rates of p53 in the normal group，paracancer group，and gallbladder cancer group were 0，12.5% and 72.5%，respectively.The difference between the three groups was statistically significant（P<0.05）.The positive expression rates of Ki67 were 2.5%，17.5% and 80%，respectively（P<0.05）.The positive expression rates of Ecadherin were 95.0%，85.0% and 30.0%，respectively（P<0.05）.The expression of CD34（MVD mean）in gallbladder carcinoma tissue（57.43±5.21）was higher than that in normal group（30.68±4.59）and paracancer group（32.66±4.28），the difference was statistically significant（P<0.05）.The results of immunohistochemistry showed that the positive expression of p53，Ki67，Ecadherin and CD34 were significantly correlated with the degree of differentiation and lymph node metastasis of gallbladder carcinoma.There was no correlation with the patient's age，gender，tumor diameter and tumor location，and the difference was not statistically significant（P>0.05）.In the TNM stage，only CD34（MVD mean）showed significant difference（P<0.05），p53，Ki67，Ecadherin and TNM staging were not correlated（P>0.05）.〖WTHZ〗Conclusion〖WTBZ〗〓p53，Ki67，Ecadherin and CD34 are closely related to the invasion and metastasis of gallbladder cancer cells，but further validation by clinical big data research is still needed.