JOURNAL OF CLINICAL SURGERY ›› 2023, Vol. 31 ›› Issue (10): 920-924.doi: 10.3969/j.issn.1005-6483.2023.10.006

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Effect of LIM kinase 1 on the proliferation,migration and ability to promote angiogenesis of hepatocellular carcinoma cells

  

  1. Department of Gastroenterology,Zhongnan Hospital of Wuhan University,Hubei Clinical Center of Intestinal and Colorectal Diseases,Hubei Key Laboratory of Intestinal and Colorectal Diseases,Hubei,Wuhan 430071,China
  • Received:2022-12-26 Accepted:2022-12-26 Online:2023-10-20 Published:2023-10-20

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Abstract: Objective   To explore the expression of LIM kinase 1(LIMK1) in human hepatocellular carcinoma cell line Huh7 and its effects on the proliferation,migration and ability to promote angiogenesis of Huh7 cells.  Methods   The expression of LIMK1 mRNA and protein in human normal hepatocyte line MIHA and human hepatoma cell line Huh7 was detected.After targeted siRNA transfection of Huh7 cells,the effects on the proliferation,migration and angiogenesis ability of hepatocellular carcinoma cells were investigated by CCK-8 assay,clone formation assay,wound healing assay,Transwell assay and endothelial cell tube formation assay.  Results   LIMK1 was highly expressed in Huh7 cells.The proliferation and migration ability of Huh7 cells were significantly decreased after knocking down the expression level of LIMK1.Human umbilical vein endothelial cells(HUVECs) have reduced in vitro angiogenic capacity.  Conclusion   LIMK1 was highly expressed in Huh7 cells,and knockdown of LIMK1 inhibited the proliferation,migration and ability to promote angiogenesis of Huh7 cells.

Key words: LIM domain kinase 1, hepatocellular carcinoma, angiogenesis, proliferation, migration

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