Abstract: Objective To analyze the relationship between the expression of transcription factors E2F2 and mucin-like receptor 2(EMR2) in colorectal cancer(CRC) tissues and the clinicopathological characteristics of patients,and to evaluate the diagnostic value of the two in cancer metastasis. Methods CRC tissue specimens and adjacent tissue specimens of CRC patients were collected during surgery,88 cases in each,real-time fluorescence quantitative PCR was used to detect the expression levels of E2F2 mRNA and EMR2 mRNA in all tissues.The expression of E2F2 protein and EMR2 protein in tissues was detected by Streptomycetin antibiotin protein-catalase linkage method(SP method),and the relationship between E2F2 protein and clinicopathological features was analyzed,and ROC curve was used to analyze the diagnostic value of E2F2 mRNA and EMR2 mRNA expression levels for lymph node metastasis in CRC patients.Results The positive rates of E2F2 protein and EMR2 protein in CRC tissues(76.50%,65.50%) were significantly higher than those in adjacent tissues(20.45%,26.14%)(P＜0.05).The expression of E2F2 protein and EMR2 protein in CRC tissue was correlated with TNM stage,lymph node metastasis and infiltration depth(P＜0.05).Compared with patients without lymph node metastasis,the expression levels of E2F2 mRNA and EMR2 mRNA in CRC tissue of patients with lymph node metastasis were obviously increased(P＜0.05).The ROC curve was drawn,and it was found that the AUC of E2F2mRNA and EMR2mRNA expression levels in CRC tissue alone and in the diagnosis of lymph node metastasis was 0.782,0.844,and 0.877,respectively.The AUC of E2F2mRNA combined with EMR2mRNA in diagnosis of lymph node metastasis was higher than that of E2F2mrna combined with EMR2mrna separately(P＜0.05). Conclusion E2F2 and EMR2 are related to TNM stage,lymph node metastasis and depth of invasion in CRC patients,and the combination of the two has certain diagnostic value for lymph node metastasis.

Key words: colorectal cancer, transcription factor E2F2, mucin-like receptor 2, lymph node metastasis, diagnosis