Abstract: Objective To investigate the effects of Sevoflurane on proliferation,apoptosis,invasion and migration,glycolysis and expression of BACE1 antisense RNA(BACE1-AS) in hepatocellular carcinoma(HCC)cells.
Methods HCC cells were cultured and treated with 4% Sevoflurane.The Cancer Genome Atlas(TCGA) was used to mine the relevant biological information of BACE1-AS,including expression in HCC tissue,prognosis correlation analysis and so on.
Results Our date showed that Sevoflurane significantly decreased the survival rate of HepG2 cells(P＜0.05).In addition,Sevoflurane was an effective pro-apoptotic factor(P＜0.05).Transwell showed that Sevoflurane decreased the invasiveness of HCC cells(P＜0.05).The scratch test showed that the migration of HepG2 cells decreased after Sevoflurane treatment,with decreased glucose uptake and lactic acid production(P＜0.05).Meanwhile,the extracellular acidified(ECAR) decreased significantly,while the oxygen consumption(OCR) increased(P＜0.05).The expression of BACE1-AS in HCC tissues was significantly higher than that in control(P＜0.05).In addition,prognostic analysis showed that the survival time of patients with high expression of BACE1-AS was significantly shorter，the hazard ratio was 1.75(P＜0.05).The expression of BACE1-AS in three kinds of HCC cells was significantly higher than that in normal hepatocytes(P＜0.05),but the expression of BACE1-AS in hepatoma cells decreased significantly after Sevoflurane treatment(P＜0.05).
Conclusion BACE1-AS is an oncogene in HCC.Sevoflurane can inhibit the proliferation,invasion,migration,glycolysis and apoptosis of HCC cells by inhibiting the expression of BACE1-AS.Sevoflurane is expected to be used as an adjuvant therapy for HCC.

Key words: hepatocellular carcinoma, Sevoflurane, invasion and migration, glycolysis, non-coding RNA