Abstract: Objection：To observe the effect on naked mice，sliver hyperplasia and hepatocellular carcinoma growth after liver arterial ligation combined with partial portal ligation.Methods：To take healthy 4weekold naked mice,establish hepatocellular carcinoma models,divide 120 naked mice into 4 groups randomly:control group(Sham n=30);partial portal vein ligation group,(pPVL n=30),hepatic artery ligation group(HAL n=30)，HAL combined with pPVL group(HAL+pPVL n=30).After corresponding treatment, serum biochemical indexes, preserved liver regeneration rate, tumor size and surrounding microvascular invasion were observed at different time points.Result：One naked mouse dead in pPVL+HAL group in 72h,also one naked mouse in pPVL group dead in 24h.The ALT and AST levels of pPVL group and HAL+pPVL group was significantly different from that of Sham group at 24h and 72h after operation(P<0.05).The preserved side liver quality of PVL group and HAL+pPVL group was significantly different from that of Sham group and HAL group at 72h,1W,2W,3W after operation(P<0.05).The tumor volume of HAL group and HAL+pPVL group was significantly different from that of PVL group and Sham group at 1W,2W and 3W after operation(P<0.05).The Sham group and PVL group were combined as the control group,and the HAL group and HAL+pPVL group were combined as the experimental group,The positive rate of MVI in the experimental group was significantly different from the control group at 3W after operation(P<0.05),and also that was significant difference between 2W and 3W after operation in the experimental group(P<0.05).Conclusion：HAL+pPVL could promote the growth of preservated liver hyperplasia and inhibit the growth of tumor in a short period of time,which provides a new treatment for some patients with advanced hepatocellular carcinoma.

Key words: hepatocellular carcinoma, hepatic artery ligation, partial portal ligation, animal experiment