Abstract: Objective：To investigate the effects of total thoracoscopic surgery on tumor specific neuron specific enolase(NSE),procalcitonin(PCT),immune markers CD3+,CD4+ and CD8+ in lung cancer patients and their correlation with prognosis.Methods：A total of 85 patients with lung cancer who underwent total thoracoscopic surgery in our hospital from October 2016 to February 2018 were selected as the observation group.102 healthy people who were enrolled in our hospital for the same period were selected as control group.The levels of serum NSE and PCT in the observation group before and after operation and in the control group were measured by electrochemiluminescence method.The levels of CD3+,CD4+ and CD8+ in the observation group before and after operation and in the control group were detected by automatic flow cytometry.Results：The NSE and PCT levels in the observation group were significantly higher than those in the control group before and after surgery,while the NSE and PCT levels after operation were significantly lower than those before surgery(P＜0.05).The proportion of CD8+ before and after operation was significantly higher than that of the control group,while the proportions of CD3+,CD4+ and CD4+/CD8+ were significantly lower than those of the control group.Compared with preoperative,the proportions of CD3+,CD4+,and CD4+/CD8+ were significantly increased after surgery(P＜0.05).The logistic regression analysis showed that NSE(OR=3.32,95%CI=1.88~6.02,P=0.023),PCT(OR=8.12,95%CI=3.25~20.12,P=0.012),CD3+(OR=2.33,95%CI=1.52~7.92,P=0.021),CD4+(OR=5.53,95%CI=3.20~8.65,P=0.018) and CD4+/CD8+(OR=4.82,95%CI=1.57~8.51,P=0.015) were independent risk factor for the prognosis of lung cancer patients undergoing thoracoscopic surgery.Conclusion：The total thoracoscopic surgery can effectively reduce the levels of NSE and PCT,improve postoperative immune function,and monitor the levels of NSE,PCT,CD3+,CD4+ and CD8+ after operation has an important role in assessing the prognosis of lung cancer.

Key words: total thoracoscopic surgery, lung cancer, neuronspecific enolase, procalcitonin, immune function