Abstract: Objective:To explore changes of serum IgA，SAA，CCL5 and inflammatory cytokines in patients with anal fistula before and after operation and their relationship with anal function and efficacy.Methods:114 anal fistula patients admitted to our hospital from October 2017 to January 2019 were selected as research objects，all of whom received surgical treatment.The serum levels of IgA，SAA，CCL5 and IL4，IL6 and IL10 were detected by enzymelinked immunosorption method.The changes in serum levels of IgA，SAA，CCL5 and IL4，IL6 and IL10 and the anal function scores of patients before and after surgery were compared to explore the relationship between serum IgA，SAA，CCL5 and IL4，IL6 and IL10 and the anal function and surgical effect of patients.Results:After treatment，the levels of IgA，SAA，CCL5，IL4，IL6，IL10 and Wexner scores of the patients were significantly decreased（P<0.05），and the differences were statistically significant（P<0.05）.Postoperative effects of IgA，SAA and CCL5 patients in the low level group were better than those in the high level group，with statistically significant differences（P<0.05）.Patients in the lowlevel IL4，IL6 and IL10 group were better than those in the highlevel group，with statistically significant differences（P<0.05）.IgA，SAA，CCL5，IL4，IL6 and IL10 were positively correlated with the course of disease and Wexner score（P<0.05），and negatively correlated with the treatment effect（P<0.05），but had no significant correlation with gender or age（P>0.05）.Conclusion:The serum levels of IgA，SAA，CCL5 and inflammatory factors in patients with anal fistula after surgery are all lower than those before surgery.The serum levels of IgA，SAA，CCL5 and inflammatory factors are closely related to the operation effect and postoperative anal function of patients.

Key words: anal fistula, IgA, serum amyloid A, chemotactic factor 5, anal function