Abstract: Objective：To investigate the antimicrobial resistance pattern of main pathogens in male inpatients with urinary tract infection in department of urology，provide clinical evidences for clinicians to prescribe the appropriate antibiotics and prevent antibiotic resistance.Methods： Analyze the medical records of 2168 cases of male inpatients retrospectively，including age，disease，blood routine examination，urine examination，urine culture and antimicrobial susceptibility test.Results：A total of 2168 cases of patients were collected，of which 402 cases with positive urine culture，the urine culture positive rate was 18.54%.The main pathogens of urinary tract infection are Escherichia coli（37.06%），Enterococcus faecalis（19.9%），Pseudomonas aeruginosa（7.96%），Klebsiella pneumoniae（7.46%），Candida albicans（5.22%）.For Escherichia coli most susceptibility was to Tigecycline，meropenem，imipenem and the most resistance to Ampicillin，piperacillin，cefazolin.For Enterococcus faecalis most susceptibility was to Fosfomycin，teicoplanin，Tigecycline and the most resistance to erythromycin.Conclusion：The pathogens of urinary tract infection are mainly Escherichia coli and Enterococcus faecalis.In clinical work，we can give empirical antibiotic therapy based on the distribution of pathogens in this region or the hospital before we have the results of urine culture，and adjust antibiotic therapy according to the results of urine culture.

Key words: urinary surgery, male, urinary tract infection, pathogens, antimicrobial resistance