关键词: 七氟烷； 微小RNA-96-5p/盐诱导激酶1轴； 胃癌细胞； 增殖； 凋亡

Abstract: Objective The objective of this study was to investigate the impacts of sevoflurane on the proliferation,invasion and apoptosis of gastric cancer (GC) cells by regulating the expression of microRNA (miR)-96-5p/salt induced kinase 1 (SIK1) axis.Methods GC cell SGC7901 cells were treated with sevoflurane of 0%,1%,2%,3%,4%,5% v/v concentration,the proliferation of SGC7901 cells was detected by CCK-8 method;SGC7901 cells were randomly grouped into three groups:control group(Normal culture),sevoflurane group(3% v/v sevoflurane intervention),sevoflurane+miR-NC group(Transfected with miR-NC and then treated with sevoflurane at 3% v/v concentration for 48h),sevoflurane+miR-96-5p mimics group(After transfection with miR-96-5p mimics, intervention was performed at 3% v/v concentration of sevoflurane for 48h),RT-qPCR was applied to detect the expression of miR-96-5p in cells of each group;flow cytometry was applied to detect apoptosis;scratch test was applied to detect cell migration;Transwell cell test was applied to detect cell invasion;Western blot was applied to detect the expression of phosphorylate-induced kinase 1 (SIK1),matrix metalloproteinase-2 (MMP-2) and MMP-9;double luciferase reporter gene experiment was applied to verify the targeting relationship between miR-96-5p and SIK1.Results Compared with the 0% concentration group,the inhibition rate of SGC7901 cell proliferation was obviously increased in a dose-dependent manner under the treatment of 1%,2%,3%,4%,and 5% v/v concentration of sevoflurane (P<0.05),the 3% v/v concentration of sevoflurane was selected for subsequent experiments;compared with the control group,the expression level of miR-96-5p,migration,invasion,and the expression levels of MMP-2 and MMP-9 proteins in sevoflurane group were obviously decreased,the apoptosis rate and the expression level of SIK1 protein were obviously increased (P<0.05);activation of miR-96-5p expression weakened the inhibition of sevoflurane on the migration and invasion of SGC7901 cells,and decreased the apoptosis rate (P<0.05);the results of double luciferase reporter gene showed that miR-96-5p could target the expression of SIK1.Conclusion Sevoflurane may inhibit the proliferation,migration and invasion of GC SGC7901 cells and promote cell apoptosis by down-regulating miR-96-5p and up-regulating SIK1.

Key words: sevoflurane; microRNA-96-5p/salt induced kinase 1 axis; gastric cancer cells; proliferation; apoptosis