临床外科杂志 ›› 2023, Vol. 31 ›› Issue (6): 542-545.doi: 10.3969/j.issn.1005-6483.2023.06.012

• 论著 • 上一篇    下一篇

结直肠癌组织转录因子E2F2、黏蛋白样受体2水平与病人临床病理特征的关系及对淋巴结转移的诊断价值

  

  1. 621000 电子科技大学医学院附属绵阳医院（绵阳市中心医院） 普外科
  • 收稿日期:2022-08-15 出版日期:2023-06-20 发布日期:2023-06-20
  • 基金资助:
    四川省医学会科研课题(S20021)

Relationship between transcription factor E2F2 and mucinlike receptor 2 levels in colorectal cancer and clinicopathological features and their diagnostic value for lymph node metastasis

  1. Department of General Surgery,Mianyang Hospital,Affiliatde to the School of Medicine，University of Electronic Science and Technology，Mianyang Central Hospital ,Sichuan,Mianyang 621000, China
  • Received:2022-08-15 Online:2023-06-20 Published:2023-06-20

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摘要： 目的 分析转录因子E2F2和黏蛋白样受体2(EMR2)在结直肠癌(CRC)组织中的表达及其与病人临床病理特征的关系，评估二者对癌转移的诊断价值。方法 2018年1月~2020年12月间住院手术的CRC病人术中切除CRC组织标本及癌旁组织标本各88例；采用实时荧光定量PCR法检测所有组织E2F2 mRNA、EMR2 mRNA表达水平；用链霉菌抗生物素蛋白过氧化氢酶连结法(SP法)检测组织中E2F2蛋白、EMR2蛋白表达，分析其与临床病理特征的关系；ROC曲线分析E2F2 mRNA、EMR2 mRNA表达水平对CRC病人淋巴结转移的诊断价值。结果 CRC组织中E2F2蛋白、EMR2蛋白阳性率(76.50%、65.50%)高于癌旁组织(20.45%、26.14%),差异有统计学意义(P＜0.05)；CRC组织E2F2蛋白、EMR2蛋白表达与病人TNM分期、淋巴结转移及浸润深度相关(P＜0.05)；与无淋巴结转移病人比较，有淋巴结转移病人CRC组织中E2F2mRNA、EMR2mRNA表达水平升高，差异有统计学意义(P＜0.05)；绘制ROC曲线发现，CRC组织中E2F2mRNA、EMR2mRNA表达水平单独及联合诊断淋巴结转移的AUC分别为0.782、0.844、0.877，E2F2mRNA联合EMR2mRNA诊断淋巴结转移的AUC高于二者分别单独诊断(P＜0.05)。结论 E2F2、EMR2与CRC病人TNM分期、淋巴结转移及浸润深度相关，二者联合对淋巴结转移具有一定诊断价值。

关键词: 结直肠癌, 转录因子E2F2, 黏蛋白样受体2, 淋巴结转移, 诊断

Abstract: Objective To analyze the relationship between the expression of transcription factors E2F2 and mucin-like receptor 2(EMR2) in colorectal cancer(CRC) tissues and the clinicopathological characteristics of patients,and to evaluate the diagnostic value of the two in cancer metastasis. Methods CRC tissue specimens and adjacent tissue specimens of CRC patients were collected during surgery,88 cases in each,real-time fluorescence quantitative PCR was used to detect the expression levels of E2F2 mRNA and EMR2 mRNA in all tissues.The expression of E2F2 protein and EMR2 protein in tissues was detected by Streptomycetin antibiotin protein-catalase linkage method(SP method),and the relationship between E2F2 protein and clinicopathological features was analyzed,and ROC curve was used to analyze the diagnostic value of E2F2 mRNA and EMR2 mRNA expression levels for lymph node metastasis in CRC patients.Results The positive rates of E2F2 protein and EMR2 protein in CRC tissues(76.50%,65.50%) were significantly higher than those in adjacent tissues(20.45%,26.14%)(P＜0.05).The expression of E2F2 protein and EMR2 protein in CRC tissue was correlated with TNM stage,lymph node metastasis and infiltration depth(P＜0.05).Compared with patients without lymph node metastasis,the expression levels of E2F2 mRNA and EMR2 mRNA in CRC tissue of patients with lymph node metastasis were obviously increased(P＜0.05).The ROC curve was drawn,and it was found that the AUC of E2F2mRNA and EMR2mRNA expression levels in CRC tissue alone and in the diagnosis of lymph node metastasis was 0.782,0.844,and 0.877,respectively.The AUC of E2F2mRNA combined with EMR2mRNA in diagnosis of lymph node metastasis was higher than that of E2F2mrna combined with EMR2mrna separately(P＜0.05). Conclusion E2F2 and EMR2 are related to TNM stage,lymph node metastasis and depth of invasion in CRC patients,and the combination of the two has certain diagnostic value for lymph node metastasis.

Key words: colorectal cancer, transcription factor E2F2, mucin-like receptor 2, lymph node metastasis, diagnosis

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