关键词: 未破裂颅内动脉瘤, 血清, miR-448-3p, Krüpple样因子5, 临床意义

Abstract: Objective To detect the expression of serum miR-448-3p and Krüpple-like factor 5 (KLF5) in patients with unruptured intracranial aneurysms and to explore their clinical significance.Methods A total of 143 patients with unruptured intracranial aneurysm (observation group) admitted to our hospital from September 2017 to May 2020 were selected as the research objects,and 150 healthy patients (control group) during the same period of physical examination were selected as controls.The expression levels of serum mir-448-3p and KLF5 mRNA,serum IL-6 and TNF were detected-α level;The t-test compared the serum miR-448-3p,KLF5 mRNA levels between the two groups;Pearson method was used to analyze the correlation between serum miR-448-3p,KLF5 mRNA levels and serum IL-6,TNF-α levels in patients with unruptured intracranial aneurysm,and the correlation between serum miR-448-3p and serum KLF5 mRNA levels.ROC curve analysis of the diagnostic value of serum miR-448-3p,KLF5 mRNA expression levels for unruptured intracranial aneurysm.Results Compared with the control group,the level of serum miR-448-3p in patients with unruptured intracranial aneurysms in the observation group were significantly reduced,and the levels of KLF5 mRNA,IL-6,and TNF-α were significantly increased (P＜0.05);compared with the number of tumors =1,the level of serum miR-448-3p in patients with unruptured intracranial aneurysms with the number of tumors ≥2 was significantly reduced (P＜0.05),and the level of KLF5 mRNA was increased (P＜0.05);compared with tumor diameter <7 mm,the level of serum miR-448-3p in patients with unruptured intracranial aneurysms with tumor diameter ≥ 7 mm were significantly reduced (P<0.05),and the level of KLF5 mRNA was significantly increased (P<0.05);the serum miR-448-3p level in patients with unruptured intracranial aneurysms was negatively correlated with serum KLF5 mRNA,IL-6,and TNF-α levels (r=-0.429,-0.442,-0.471,P＜0.05),and the serum KLF5 mRNA level was positively correlated with serum IL-6 and TNF α levels (r=0.454,0.523,P＜0.05).The area under the ROC curve of the serum miR-448-3p,KLF5 mRNA expression level for the diagnosis of unruptured intracranial aneurysm was 0.978 and 0.995,sensitivity was 93.7% and 95.8%,and the specificity was 90.7% and 98.7%,respectively.Conclusion The serum miR-448-3p level of patients with unruptured intracranial aneurysm is decreased,and the KLF5 level is increased.Both levels are closely related to the number of tumors,tumor diameter,and inflammation levels in patients,and may jointly participate in the progression of the aneurysm.

Key words: unruptured intracranial aneurysm, serum, miR-448-3p, Krüpple-like factor 5, clinical significance