阿帕替尼对结肠癌细胞侵袭能力的影响及其分子机制

doi:10.3969/j.issn.1005-6483.2022.02.024

临床外科杂志 ›› 2022, Vol. 30 ›› Issue (2): 179-181.doi: 10.3969/j.issn.1005-6483.2022.02.024

阿帕替尼对结肠癌细胞侵袭能力的影响及其分子机制

437199 湖北省咸宁市中心医院湖北科技学院附属第一医院胃肠外科Ⅱ病区

收稿日期:2021-05-17 接受日期:2021-05-17 出版日期:2022-02-20 发布日期:2022-02-20
通讯作者: 田晓玲，Email:tianxl2005@163.com

Apatinib reduces the invasion ability of colon cancer cells and its molecular mechanism

Department of Gastrointestinal Surgery,Ward II,the First Affiliated Hospital of Hubei University of Science and Technology,Xianning Central Hospital,Hubei Province,Xianning 437199,China

Received:2021-05-17 Accepted:2021-05-17 Online:2022-02-20 Published:2022-02-20

摘要/Abstract

摘要： 目的观察阿帕替尼对结肠癌细胞株SW480侵袭能力及间质上皮转化(mesenchymal-epithelial transition，MET)的影响及其分子机制。方法通过细胞存活实验筛选阿帕替尼适宜剂量，通过Transwell检测其对细胞侵袭能力的影响，通过Western blot检测MET相关蛋白的变化。结果在SW480中加入不同浓度的阿帕替尼，观察到当阿帕替尼的浓度≤120nM时，细胞的存活能力没有明显的改变。因此后续实验中选择60nM及120nM两种药物剂量。60nM及120nM的阿帕替尼作用下，SW480细胞侵袭能力降低，并且MET相关的Ecadherin表达升高，而Vimentin、Snail及Slug的表达降低。结论阿帕替尼可诱导结肠癌细胞株SW480由间质表型向上皮表型转化，并抑制细胞的侵袭能力。

关键词: 阿帕替尼, 结肠癌, 侵袭, 间质-上皮转化

Abstract: Objective To observe the effect of apatinib on the invasion ability and mesenchymal-epithelial transition(MET) of colon cancer cell line SW480 and its molecular mechanism. Methods Different doses of apatinib were incubated with the SW480 cells,then the effect of apatinib on the invasion of SW480 cells was detected by Transwell assay,and the expression of MET related proteins were detected by Western blot. Results The cell viability of SW480 was remain the same when the concentration of apatinib was ≤ 120nM,thus 60nM and 120nM apatinib were used in the further experiments.The invasion of SW480 cells was reduced after apatinib incubation for 48h,and the expression of E-cadherin was enhanced,with the depresion of Vimentin，Snail and Slug.Conclusion Apatinib could induce the MET,and reduced the invasion ability of SW480 cells.

Key words: Apatinib, colon cancer, invasion, mesenchymal-epithelial transition

朱栋良黎尉浩严海东尹小平田晓玲. 阿帕替尼对结肠癌细胞侵袭能力的影响及其分子机制[J]. 临床外科杂志, 2022, 30(2): 179-181.

ZHU Dongliang, LI Weihao, YAN Haidong, et al. Apatinib reduces the invasion ability of colon cancer cells and its molecular mechanism[J]. JOURNAL OF CLINICAL SURGERY, 2022, 30(2): 179-181.

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阿帕替尼对结肠癌细胞侵袭能力的影响及其分子机制

Apatinib reduces the invasion ability of colon cancer cells and its molecular mechanism

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