miR-597调控FOSL2抑制乳腺癌细胞增殖、迁移及侵袭的研究

doi:10.3969/j.issn.1005-6483.2021.03.013

摘要/Abstract

摘要： 目的探讨miR-597对乳腺癌细胞增殖、迁移及侵袭的影响，及其与FOS 样抗原 2(Fos-related antigen 2,FOSL2)的靶向关系。
方法选取乳腺癌细胞株T-47D,SK-BR-3,MDA-MB-231,MCF7及BT-474，正常乳腺细胞株MCF10A为研究对象，分别转染miR-597（miR-597组）及 RNA 阴性对照序列（NC组），同时选取30例乳腺癌病人癌组织及癌旁正常乳腺组织，迅速液氮冻存以提取RNA用于qRT-PCR检测。采用实时荧光定量 PCR 法检测细胞、组织中miR-597表达水平；MTT、流式细胞术检测细胞增殖能力；Transwell小室实验检测细胞迁移、侵袭能力；Western blot检测细胞FOSL2表达水平；生物信息预测miR-597与FOSL2的靶向作用关系，荧光素酶实验验证。
结果乳腺癌组织miR-597水平低于正常乳腺组织，乳腺癌细胞株细胞miR-597表达低于正常乳腺细胞株细胞，差异有统计学意义(P＜0.01)；miR-597组细胞增殖能力明显低于NC组，差异有统计学意义(t=9.833，P=0.003)，miR-597组G0G1期细胞百分率高于NC组，S期细胞百分率低于NC组(t=10.215，10.336，P=0.000，0.000)；miR-597组细胞侵袭能力低于NC组(t=8.404，P=0.000)，miR-597组细胞迁移能力低于NC组(t=5.801，P=0.016)；miR-597组细胞FOSL2表达水平低于NC组(t=13.658，P=0.000)；miR-597与FOSL2有良好的靶向关系。
结论 miR-597在乳腺癌组织及细胞株中低表达，其高表达可可抑制乳腺癌细胞的增殖、迁移及侵袭能力，其机制与调控FOSL2有关。

关键词: miR-597, FOS样抗原2, 乳腺癌, 转移, 侵袭

Abstract: Objective To investigate the effect of miR-597 on the proliferation,migration and invasion of breast cancer cells,and its relationship with FOS related antigen 2(FOSL2).
Methods Breast cancer cell lines T-47D,SK-BR-3,MDA-MB-231,MCF7,BT-474 and normal breast cell line MCF10A were selected as the study objects.According to the transfection,it was divided into:NC group(null plasmid) and miR-597 group(simulated sequence).Meanwhile,30 patients with breast cancer tissue and normal breast tissue adjacent to cancer were selected，rapid cryopreservation of liquid nitrogen to extract RNA for qRT-PCR.The expression level of miR-597 in cells and tissues was detected by real-time fluorescence quantitative PCR.The cell proliferation was detected by MTT and flow cytometry.The cell migration and invasion were detected by Transwell chamber.The expression of FOSL2 was detected by Western blot.The target effect of miR-597 and FOSL2 was confirmed by bioinformatics methodsand the luciferase reporter assay.
Results The expression of miR-597 in breast cancer tissue was lower than that in normal breast tissue(P<0.01).The cell proliferation ability of miR-597 in breast cancer cell line was lower than that in normal breast cell line(P<0.01).The percentage of G0G1 phase cells in miR-597 group was higher than that in NC group,and the percentage of S-phase cells was lower than that in NC group(t=10.215,10.336,P=0.000,0.000).The cell invasion ability of miR-597 group was lower than that of NC group(t=8.404,P=0.000).The cell migration ability of miR-597 group was lower than that of NC group(t=5.801,P=0.016).The expression level of FOSL2 in miR-597 group was lower than that of NC group(t=13.658,P=0.000).MiR-597 had a good targeting relationship with FOSL2.
Conclusion miR-597 was low expressed in breast cancer tissue and cell lines.The high expression of miR-597 can inhibit the proliferation,migration and invasion of breast cancer cells,and which was related to the regulation of FOSL2.

Key words: miR-597, Fos like antigen 2, breast cancer, metastasis, invasion

周蓓王婧李仲洋. miR-597调控FOSL2抑制乳腺癌细胞增殖、迁移及侵袭的研究[J]. 临床外科杂志, 2021, 29(3): 248-252.

ZHOU Bei, WANG Jing, LI Zhongyang.. miR-597 inhibits breast cancer cell proliferation,migration and invasion through FOSL2[J]. JOURNAL OF CLINICAL SURGERY, 2021, 29(3): 248-252.

参考文献

相关文章 15

[1]	蔡耿喜刘强. 乳腺癌的精准治疗[J]. 临床外科杂志, 2021, 29(3): 201-207.
[2]	刘健吴凡. 精准医学对乳腺癌化疗方案选择的积极意义[J]. 临床外科杂志, 2021, 29(3): 208-211.
[3]	王朝斌王殊. 乳腺癌保留腋窝的临床试验进展[J]. 临床外科杂志, 2021, 29(3): 212-214.
[4]	马天怡王海波. 肿瘤整形技术在保乳手术中的应用与进展[J]. 临床外科杂志, 2021, 29(3): 215-217.
[5]	黄春旺丛淑珍. 非典型乳腺癌超声诊断的要点及难点[J]. 临床外科杂志, 2021, 29(3): 218-221.
[6]	任敏徐云凤. 乳腺癌乳房成形手术方法的精准选择[J]. 临床外科杂志, 2021, 29(3): 222-225.
[7]	戴嘉婧韩馨乐钟福波林哲广江淑琪韦伟方征宇. 三阴性乳腺癌与癌旁组织中差异表达环状RNA筛选及生物信息学分析[J]. 临床外科杂志, 2021, 29(3): 227-230.
[8]	杨丽朱荔刘紫朦鲍宝石马金平王建东. 超声引导下空芯针穿刺活检对乳腺癌的诊断价值[J]. 临床外科杂志, 2021, 29(3): 231-234.
[9]	聂丽静奚凯雯韦嘉玲董晓晶方琼金玉翡. 个案管理模式下乳腺癌病人辅助内分泌治疗依从性分析[J]. 临床外科杂志, 2021, 29(3): 235-238.
[10]	黄雅静吴昊罗智勇. 乳腺癌术后一步法与二步法重建乳房的临床效果分析[J]. 临床外科杂志, 2021, 29(3): 239-242.
[11]	张振伟孙家和张立功钱军. 乳腺癌骨转移病人危险因素及预后因素分析[J]. 临床外科杂志, 2021, 29(3): 243-247.
[12]	段书强鲍方刘振邦王菲高慧敏开蕾. 趋化因子配体12/趋化因子受体4轴在三阴性乳腺癌组织中表达及与淋巴结转移关系[J]. 临床外科杂志, 2021, 29(3): 254-257.
[13]	闫军周高晋. 乳腺癌外周血单核细胞自噬相关基因表达及其与肿瘤复发的关系[J]. 临床外科杂志, 2021, 29(3): 258-260.
[14]	李颖董明华张萌萌孟芳杨晓慧. 低频超声微泡增强紫杉醇诱导三阴性乳腺癌细胞自噬的作用及机制[J]. 临床外科杂志, 2021, 29(3): 261-264.
[15]	李金穗周瑶蒲卢兰徐明飞侯令密高砚春. 混合核苷片预防乳腺癌表柔比星+环磷酰胺方案辅助化疗所致骨髓抑制的临床研究[J]. 临床外科杂志, 2021, 29(3): 265-268.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed