关键词: 膀胱癌, miR-143-3p, ATG2B, 临床病理特征, 预后

Abstract: Objective To investigate the expression of miR-143-3p and ATG2B in serum of patients with bladder cancer and their relationship with clinicopathology,prognosis and survival of patients.Methods Eighty-eight patients who underwent radical bladder cancer surgery in our hospital from January 2017 to January 2019 were regarded as the research group,and 80 volunteers who underwent physical examination in our hospital were included as the control group.Quantitative real-time PCR (qRT-PCR) method was performed to detect the levels of miR-143-3p and ATG2B;The predictive value of miR-143-3p and ATG2B for bladder cancer was evaluated by ROC curve.Draw survival curve using Kaplan Meier method;The prognostic factors of bladder cancer patients were analyzed by Cox regression.Results The expression level of miR-143-3p in the serum of bladder cancer patients was lower than that in the control group (0.68±0.12 vs. 0.87±0.26),and the expression level of ATG2B was higher (1.02±0.30 vs. 0.61±0.18) (P<0.05);the expression level of miR-143-3p in serum of postoperative patients was higher than that before operation (0.77±0.23 vs. 0.68±0.12),and the expression level of ATG2B was lower than that before operation (0.85±0.26 vs. 1.02±0.30) (P<0.05);The study group showed a negative correlation between miR-143-3p and ATG2B expression (r=-0.454,r=-0.407,P<0.01);The AUC of miR-143-3p in the diagnosis of bladder cancer was 0.846 (95%CI:0.783-0.897),the sensitivity and specificity were 79.55% and 78.75% respectively;The AUC of ATG2B in the diagnosis of bladder cancer was 0.883 (95%CI:0.824-0.927),the sensitivity and specificity were 78.41% and 81.25% respectively;The AUC for the diagnosis of bladder cancer was 0.939 (95%CI:0.891-0.970),the sensitivity and specificity were 87.50% and 88.75%,respectively.The 3-year survival rate of the miR-143-3p low expression group was 34.88% (15/43) lower than that of the high expression group 77.78% (35/45),and the 3-year survival rate of the ATG2B high expression group was 40.91% (18/44) lower than that of the low expression group 72.73% (32/44).The differences were statistically significant (Log rank χ²=18.055,8.658,P=0.000,0.003).Lymph node metastasis,distant metastasis,tumor pathological grade,TNM stage,miR-143-3p,ATG2B are all factors influencing the survival of bladder cancer patients.Conclusion The serum expression of miR-143-3p in patients with bladder cancer is low,and the expression of ATG2B is high,and the expressions of the two are closely related to the clinicopathological characteristics and prognosis of patients with bladder cancer.

Key words: bladder cancer, miR-143-3p, ATG2B, clinicopathological features, prognosis