关键词: 腹部创伤； 感染； 基质金属蛋白酶9； 基质细胞衍生因子1； 诊断价值

Abstract: Objective To investigate the expression of matrix metalloproteinase-9 (MMP-9) and stromal cell-derived factor-1 (SDF-1) in serum of patients with abdominal trauma infection and their diagnostic value.Methods From July 2021 to July 2022,100 patients with abdominal trauma infection admitted in our hospital were regarded as the case group,100 patients with abdominal trauma without infection were regarded as the control group.Serum levels of MMP-9 and SDF-1 were detected by enzyme-linked immunosorbent assay (ELISA);correlation between serum MMP-9, SDF-1 and SIRS score and length of hospital stay were analyzed by Spearman method;the diagnostic value of serum MMP-9 and SDF-1 levels in patients with abdominal trauma infection was analyzed by ROC curve;the levels of serum MMP-9 and SDF-1 in patients with abdominal trauma infection before and after treatment were analyzed in matched samples t;multivariate Logistic regression analysis was applied to analyze the factors affecting the occurrence of abdominal trauma infection.Results The levels of serum MMP-9,SDF-1,CRP,WBC,TNF-α,diabetes and trauma types in the case group were higher than those in the control group (P<0.05).The level of serum MMP-9 in patients with abdominal trauma infection was positively correlated with SIRS score,CRP,WBC and TNF-α (r=0.505,0.471,0.423,0.416,P<0.05).Serum SDF-1 level was also positively correlated with SIRS score,CRP,WBC and TNF-α (r= 0.469,0.439,0.518,0.469,P<0.05).ROC analysis showed that the area under the curve (AUC) of serum MMP-9 and SDF-1 for predicting infection in abdominal trauma was 0.850 (95%CI:0.799-0.902) and 0.806 (95%CI:0.746-0.866),respectively.The AUC of combined detection was 0.914 (95%CI:0.876-0.951),which was significantly higher than that of MMP-9 and SDF-1 alone (Z=1.987,2.97,P<0.05).The expression levels of serum MMP-9 and SDF-1 after treatment were lower than those before treatment (P<0.05).Logistic analysis showed that diabetes,trauma type,serum levels of MMP-9 and SDF-1 were risk factors for abdominal trauma infection (P<0.05).Conclusion The levels of serum MMP-9 and SDF-1 in patients with abdominal trauma infection are increased,they may be involved in the occurrence and development of abdominal trauma infection,and have important significance for the diagnosis of abdominal trauma infection.

Key words: abdominal trauma; infection; matrix metalloproteinase 9; stromal cell-derived factor-1; diagnostic value