关键词: 急性胰腺炎； 微小RNA-325-3p； 成纤维细胞生长因子10； 病情； 预后

Abstract: Objective To investigate the value of the expression levels of serum microRNA-325-3p (miR-325-3p) and fibroblast growth factor 10 (FGF10) in evaluating the severity and prognosis of acute pancreatitis.Methods A total of 265 patients with acute pancreatitis diagnosed and treated in our hospital from June 2021 to October 2022 were selected as the observation group,the patients were divided into mild acute pancreatitis group (100 cases),moderate severe acute pancreatitis group (70 cases) and severe acute pancreatitis group (95 cases) according to clinical severity;in the same period,260 healthy volunteers served as the control group A,and 260 patients with chronic pancreatitis as control group B.According to 28 d survival condition,the severe patients were divided into survival group (n=68) and death group (n=27).To compare miR-325-3p and FGF10 levels in each group of patients.Analysis of the correlation between serum miR-325-3p,FGF10 levels and Acute Physiology and Chronic Health Evaluation Ⅱ score and the correlation between serum miR-325-3p and FGF10 in patients with acute pancreatitis;Logistic regression analysis was used to analyze the factors affecting the prognosis of patients with severe acute pancreatitis;the prognostic value of serum miR-325-3p and FGF10 levels in patients with severe acute pancreatitis was analyzed by ROC curve.Results Compared with the control group A and B,the level of miR-325-3p in serum of patients with mild,moderate and severe acute pancreatitis decreased obviously in turn (P<0.05),and the levels of FGF10 increased obviously in turn (P<0.05);serum miR-325-3p in patients with acute pancreatitis was negatively correlated with FGF10 level and APACHE Ⅱ score (APACHE Ⅱ:r=-0.664,P<0.05;FGF10:r=-0.687,P<0.05),and FGF10 was positively correlated with APACHE Ⅱ score (r=0.676,P<0.05).Compared with the survival group,the body mass index,APACHE Ⅱ score and FGF10 level in the death group were obviously higher (P<0.05),and the miR-325-3p level was obviously lower (P<0.05);Logistic regression analysis showed that body mass index,APACHE Ⅱ score and FGF10 were risk factors affecting the prognosis of patients with severe acute pancreatitis (P<0.05),miR-325-3p was a protective factor affecting the prognosis of patients (P<0.05);the area under the ROC curve (AUC) of miR-325-3p combined with FGF10 to predict the prognosis of patients with severe acute pancreatitis was 0.972,which was better than their individual prediction (Z_{combination-miR-325-3P}=1.984,P=0.047;Z_{combination-FGF10}=2.219,P=0.027).Conclusion The level of serum miR-325-3p in patients with acute pancreatitis decreases,and the level of FGF10 increases,they are related to the severity and prognosis of patients,and have certain value in evaluating the prognosis of patients.

Key words: acute pancreatitis; miR-325-3p; FGF10; disease condition; prognosis