关键词: 结直肠癌； 微小RNA-383； 微小RNA-4458； 微小RNA-93； 预后

Abstract: Objective To investigate the expression levels and clinical significance of microRNA-383(miR-383),microRNA-4458(miR-4458),and microRNA-93(miR-93) in colorectal cancer tissue.Methods From October 2018 to October 2020,124 colorectal cancer patients who underwent surgical treatment in our hospital were regarded as the colorectal cancer group,and their adjacent normal tissues were as the adjacent control group.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR) method was applied to detect the expression levels of miR-383,miR-4458,and miR-93 in tissues;the relationship between the expression of miR-383,miR-4458,and miR-93 in colorectal cancer tissue and patient prognosis was analyzed using Kaplan-Meier curve;Cox regression was applied to analyze the prognostic factors of colorectal cancer patients.Results Compared with the adjacent control group,the expression levels of miR-383,miR-4458,and miR-93 in the colorectal cancer group were obviously lower(P＜0.05).The expression of miR-383,miR-4458,and miR-93 in colorectal cancer tissue was related to clinical and pathological features such as tumor diameter,TNM staging,lymph node metastasis,and depth of invasion(P＜0.05).The three-year survival rate of patients with high expression of miR-383 in colorectal cancer tissue(55/63,87.30%) was higher than that of patients with low expression of miR-383(41/61,67.21%)(χ²=7.650,P＜0.05);the three-year survival rate of patients with high expression of miR-4458 in colorectal cancer tissue(53/60,88.33%) was higher than that of patients with low expression of miR-4458(43/64,67.18%)(χ²=8.566,P＜0.05);the three-year survival rate of patients with high expression of miR-93 in colorectal cancer tissue(51/59,86.44%) was higher than that of patients with low expression of miR-93(45/65,69.23%)(χ²=5.612,P＜0.05).According to multivariate Cox regression analysis,miR-383,miR-4458,and miR-93 are protective factors for the prognosis of colorectal cancer patients(P＜0.05),while tumor diameter,TNM staging,lymph node metastasis,and depth of infiltration were risk factors for the prognosis of colorectal cancer patients(P＜0.05).The area under the curve(AUC) predicted by Cox regression model is 0.917,95%CI is 0.854~0.959(Z=12.338,P＜0.05),the sensitivity is 94.29%,and the specificity is 87.64%.Conclusion The expression levels of miR-383,miR-4458,and miR-93 in colorectal cancer tissue are obviously reduced,and their expression levels are related to the clinical pathological characteristics and prognosis of colorectal cancer patients.

Key words: colorectal cancer; miR-383; miR-4458; miR-93; prognosis