关键词: 细胞凋亡抑制因子1, 肝癌, p53, p21

Abstract: [Abstract] Objective To investigate the effects of TP53 regulation of apoptosis inhibitor 1(TRIAP1) on the proliferation and invasion ability of hepatoma cells Huh7 and HepG2.Methods In this study,the difference of TRIAP1 expression in tumor tissues and normal tissues was analyzed using TCGA database,and the correlation between TRIAP1 and prognosis of HCC patients was analyzed.TRIAP1 knockdown cell lines were constructed by transfection with siRNA and control group.Western blot was used to verify knockdown efficiency of TRIAP1 siRNA transfection and the levels of other related proteins.The effect of TRIAP1 on the apoptosis of HCC cells was detected by Capase 3/7 active cell apoptosis kit and Annex INV /PI assay,and the effect of TRIAP1 on the proliferation of HCC cells was detected by CCK8.Results TCGA database analysis showed that TRIAP1 expression was up-regulated in HCC cells,and was negatively correlated with prognosis of HCC patients.siTRIAP1 was transfected into Huh7 and HepG2 to downregulate TRIAP1 expression..The mRNA and protein expression levels of p21 in the TRIAP1 knockdown group were significantly up-regulated,and the proliferation ability of the TRIAP1 knockdown group was decreased,while the number of apoptotic cells was increased.Conclusion The expression of TRIAP1 is up-regulated in HCC cells,and the expression of cell cycle related p21 expression is decreased and then regulate the tumor cell cycle.Inhibition of TRIAP1 can significantly up-regulate p21,inhibit the proliferation ability of HCC cells,and promote the apoptosis process.

Key words: TP53 regulation of apoptosis inhibitor 1, hepatocellular carcinoma, p53, p21