关键词: POU3F2, 胶质瘤, 转录组, 分子标志物, 生物信息学

Abstract: Objective To explore the expression and significance of POU3F2 in glioma based on the Chinese Glioma Genome Atlas(CGGA). Methods Based on CGGA,GEPIA2,String,Metascape and other databases,we analyze the differences in the expression of POU3F2 gene in different WHO grade groups of glioma with R program language.Meanwhile,IHC staining was conducted to detect the protein expression level of POU3F2 in collected glioma tumor tissues.Then the relationships between POU3F2 gene expression and molecular subtypes of glioma,molecular biomarkers and clinical prognosis were analyzed. Results The expression of POU3F2 was increased in the high grade glioma group(P=0.0037).Immunohistochemistry indicated that POU3F2 expression was also up-regulated in high grade glioma group (P<0.001).The expression levels of POU3F2 was the highest in the classical glioma,and the difference was significant between other molecular subtypes (all P<0.001),and the AUC value of POU3F2 predicted the classical glioma was 0.784.The expression levels of POU3F2 gene were significantly lower in glioma with chromosome 1p19q co-deletion.High levels of POU3F2 expression tended to have a poorer prognosis(P=0.013)and was verified in the TCGA database（P=0.0055）.Conclusion The increased expression level of POU3F2 is associated with malignant glioma phenotype,and might be a molecular biomarker of glioma.

Key words: POU3F2, glioma, transcriptome, molecular biomarker, bioinformatics