关键词: miR-367-3p, 细胞周期素D2重组蛋白, 肺癌, 增殖, 迁移

Abstract: Objective To investigate the effect of miR-367-3p regulating the expression of recombinant cyclin D2 protein(CCND2) on the proliferation and migration of lung cancer cells.Methods Human lung cancer cells Calu,MSTO-211H,NCI-H2347,NCI-H1437,NCI-H1944,NCI-H647,human normal lung cells HLF-a were selected,qRT-PCR method was used to determine the expression level of miR-367-3p in the cells.Calu cells were divided into control group,miR36-7-3p NC group,miR-367-3p mimic group,miR-367-3p mimic+pcCCND2 group,CCK-8 method was used to determine the viability of Calu cells in each group;flow cytometry was used to measure cell apoptosis ability.Transwell test was used to measure cell invasion ability.Scratch test was used to measure the migration ability of Calu cells.WB method was used to determine the relative expression of CCND2,apoptosis and invasion-related proteins in Calu cells.Luciferase assay was used to determine the targeting relationship between miR-367-3p and CCND2.Results miR-367-3p showed low expression in human lung cancer cells;compared with the control group,the Calu cell viability,invasion,migration ability,MMP-2 and MMP-9 protein expression in the miR-367-3p mimic group were significantly reduced(P＜0.05),the apoptosis ability,Caspase-3,Bax protein expression were significantly increased(P＜0.05),the Bcl-2 protein expression was significantly reduced(P＜0.05);CCND2 overexpression reversed the inhibitory effect of miR-367-3p overexpression on Calu cell viability,invasion,and migration;the results of luciferase experiments showed that miR-367-3p and CCND2 had a targeted regulatory relationship.Conclusion miR-367-3p can inhibit the proliferation and migration of human lung cancer cells Calu,which may be achieved by inhibiting CCND2.

Key words: miR-367-3p, recombinant cyclin D2 protein, lung cancer, proliferation, migration