关键词: 静脉血栓, 静脉内皮细胞, Notch信号通路

Abstract: Objective To investigate the expression regulation mechanism of miR-5189-3p in deep venous thrombosis of lower limbs through animal model test.
Methods 48 SPF SD rats were selected.They were randomly divided into 4 groups:normal control group(group A),simple inferior vena cava thrombosis model group(group B),hsa-miR-5189-3p mimics group(group C) and hsa-miR-5189-3p NC group(group D),with 12 mice in each group.DVT modeling was carried out in SD rats.24 hours after the modeling was completed,four groups of SD rats were sacrificed for cervical dislocation,and the thromboembolization of SD rats with inferior vena cava thrombosis was observed and tissue samples were collected.Pathological changes of SD rats' inferior vena cava and its contents were observed by pathological HE staining sections.Real-time fluorescence quantitative PCR was used to detect the expression differences of JAG1,Notch1 and Hes1 in inferior vena cava tissues of SD rats.
Results In the miRNA mimics group and the miRNA mimics negative control group,there were more nucleated cells in the thrombotic tissue,the degree of organization increased,and some small fissures were generated and formed in the peripheral area of thromboembolism,thus forming the lumen like structure with signs of blood flow recanalization.Real-time fluorescence quantitative PCR results of JAG1,Notch1 and Hes1 showed that after upregulation of miR-5189-3p,the expression levels of JAG1,Notch1 and Hes1 increased significantly(P<0.05).
Conclusion hsa-miR-5189-3p may be a key miRNA for deep venous thrombosis of lower extremities.

Key words: vein thrombosis, venous endothelial cells, Notch signaling pathway