临床外科杂志 ›› 2019, Vol. 27 ›› Issue (4): 331-334.doi: 10.3969/j.issn.1005-6483.2019.04.019

• • 上一篇    下一篇

绝经后骨质疏松症病人骨髓间充质干细胞STIM1与成骨分化关键因子的表达变化

  

  1. 重庆市武警总队医院骨科
  • 收稿日期:2018-05-30 出版日期:2019-04-20 发布日期:2019-04-20

The expression of STIM1 and osteogenetic differentiation related factors in bone marrow stromal stem cells of postmenopausal osteoporosis patients

  1. Department of Orthopedics，Chongqing General Team Hospital of Armed Police Force，Chongqing 400061，China
  • Received:2018-05-30 Online:2019-04-20 Published:2019-04-20

PDF (PC)

517

PDF (Mobile)

6

摘要： 目的 探讨绝经后骨质疏松症病人骨髓间充质干细胞（BMSCs）基质交感分子1（STIM1）与成骨分化关键因子碱性磷酸酶（ALP）、骨桥蛋白（OCN）、骨钙素（OPN）的表达变化情况。方法 通过临床取材获得并培养绝经后骨质疏松症病人BMSCs，加入成骨诱导液孵育14天，采用RT-PCR检测ALP、OPN、OCN的转录水平及ALP的活性，并检测STIM1的表达情况。采用ALP染色法检测ALP活性；采用Alizarin Red染色法检测钙结节形成数量；采用Fluo荧光法检测钙离子内流变化。结果 通过无菌培养，获得高纯度绝经后骨质疏松症（观察组）病人的BMSCs，经成骨诱导14天后，观察组病人BMSCs中成骨分化关键因子ALP、OPN、OCN的转录水平以及ALP的活性均低于对照组（P<0.05），同时STIM1 mRNA的表达水平也相应下降，钙离子内流较对照组减少（P<0.05）。另外，观察组病人BMSCs钙结节形成数量少于对照组。结论 绝经后骨质疏松的发病机制可能与由STIM1参与调控的钙释放激活钙离子通道开放情况密切相关，可作为绝经后骨质疏松病人预防和治疗的潜在靶点。

关键词: 绝经后骨质疏松, 骨髓间充质干细胞, 基质交感分子1, 碱性磷酸酶, 钙释放激活钙离子通道

Abstract: Objective To observe the expression of stromal interaction molecule-1（STIM1）and other osteogenetic differentiation related factors （such as ALP，OCN，OPN） in bone marrow stromal stem cells（BMSCs）of postmenopausal osteoporosis patients.Methods BMSCs were cultured in the presence of osteogenic induction for 14d after obtained from postmenopausal osteoporosis patients.We analyzed the Ca2+ concentration and Ca2+ release activated Ca2+channel（CRAC）by Fluo-3 staining before and after osteogenic induction respectively.The expressions of STIM1 and other osteogenic differentiation related factors（ALP，OPN and OCN）were further measured and evaluated by quantitative real time PCR.Mineralization was evaluated by the calcium chelating dye alizarin red at the start of osteogenic induction and after 7 and 14 days.BMSCs were stained for alkaline phosphatase（ALP）at 14d.Results BMSCs were successfully cultured after osteogenic induction for 14d.The expressions of STIM1 and other osteogenic differentiation related factors（ALP，OPN，and OCN）in observation group were lower than the control group by RT-PCR（P<0.05）.Similarly，ALP staining was significantly reduced in observation group compared with the normal control cells（P<0.05）.Besides，the expression of STIM1 in observation group after osteogenic induction for 14d was reducing，but less than control group.Ca2+ concentration was reduced in observation group than control group（P<0.05）.The numbers of calcium chelating dye alizarin red in observation group were less than control group.Conclusion There are significant evidences that STIM1 in BMSCs would be contributed to the postmenopausal osteoporosis，which could provide a new research direction and therapeutic strategy for osteoporosis.

Key words: postmenopausal osteoporosis, bone marrow mesenchymal stem cells, stromal sympathetic molecules 1, alkaline phosphatase, calcium release activated calcium channel

No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 雷霆;舒凯. 加强专科医师全面训练;不断提高神经外科手术中脑血管问题的处理水平[J]. 临床外科杂志, 0, (): 481 .
[2] 万松林;刘韦成;吴云华;等. 胃间质瘤腹腔内破裂出血一例[J]. 临床外科杂志, 2016, 24(10): 807 .
[3] 周振宇;汪路明;王一青;等. 肺部多发结节468例诊疗分析[J]. 临床外科杂志, 2016, 24(11): 865 .
[4] 高纯;胡俊波. 临床营养筛查与评估方法的现状与进展[J]. 临床外科杂志, 2016, 24(12): 896 .
[5] 殷刚;罗良弢;严想元. 加ω-3脂肪酸全肠外营养对肠梗阻术后患者肠黏膜屏障功能的影响[J]. 临床外科杂志, 2016, 24(12): 916 .
[6] 臧书源. 甲状腺腺叶切除术治疗不同年龄甲状腺良性肿瘤患者的临床疗效比较[J]. 临床外科杂志, 2016, 24(12): 931 .
[7] 钱群. 出口梗阻型便秘手术治疗风险控制 [J]. 临床外科杂志, 2016, 24(6): 413 .
[8] 吴超;汪全新;卢晓明 . 腹腔镜改良经肛门结肠肛管吻合术切除低位直肠癌保肛手术的临床疗效分析 [J]. 临床外科杂志, 2016, 24(6): 437 .
[9] 吕晶. 尼卡地平对气管插管刺激下脑电双频指数反应、收缩压和心率的影响[J]. 临床外科杂志, 2016, 24(8): 635 .
[10] 万春;万品文;戴兵. 陈氏双悬吊法在中肝叶肿瘤切除术中的应用[J]. 临床外科杂志, 2016, 24(8): 592 .
版权所有 © 《临床外科杂志》编辑部
地址：武汉市武昌区东湖路165号　邮编：430071　电话：027-87893476　E-mail: whlcwk@126.com
本系统由北京玛格泰克科技发展有限公司设计开发
鄂ICP备11011458号-3