关键词: 膀胱癌, 微小RNA-433-3p, β微管蛋白V, 病理特征, 术后复发

Abstract: Objective  To explore the relationship between the expression of microRNA-433-3p (miR-433-3p) and β microtubule V (TUBB6) in bladder cancer tissue and its pathological characteristics,and its predictive value for postoperative recurrence.Methods From August 2021 to August 2022, 116 patients with bladder cancer underwent surgery for cancer tissue and adjacent tissue,and which were divided into recurrence group (43 cases) and non recurrence group (73 cases) according to whether there was local recurrence or distant recurrence and metastasis after surgery.The quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the mRNA levels of miR-433-3p and TUBB6.The influencing factors of postoperative recurrence of bladder cancer were analyzed by logistic- regression. The predictive value of miR-433-3p and TUBB6 in postoperative recurrence of bladder cancer was analyzed by receiver operating characteristic (ROC) curve.Results  The level of miR-433-3p in cancer tissue was obviously lower than that in adjacent tissue (P<0.05),and the level of TUBB6 was obviously higher than that in adjacent tissue (P<0.05).The level of miR-433-3p was significantly reduced (P<0.05) in patients with TNM stage Ⅲ+Ⅳ,histological differentiation to low differentiation,and tumor infiltration,while the level of TUBB6 mRNA was significantly increased (P<0.05).The serum miR-433-3p level in the recurrent group was significantly lower than that in the non recurrent group,,The level of TUBB6 mRNA was higher in the non recurrent group,and the difference was statistically significant (P<0.05).TNM stage Ⅲ+Ⅳ,tumor infiltration,and high expression of TUBB6 are independent risk factors for postoperative recurrence in patients (P<0.05),while high expression of miR-433-3p is an independent protective factor (P<0.05).ROC curve analysis shows that,,the area under curve (AUC) predicted by serum miR-433-3p and TUBB6 levels in combination was significantly higher than that predicted by a single index (miR-433-3p=2.323,TUB6=1.961,P=0.020,0.048),sensitivity of 95.35%,specificity of 82.19%.Conclusion The expression of miR-433-3p and TUBB6 in bladder cancer tissues is closely related to pathological features,and the combination of the two tissues has a higher predictive value for postoperative recurrence.

Key words: bladder cancer, microRNA-433-3p, β microtubule protein V, pathological features, postoperative recurrence