结直肠癌组织中溴结构域蛋白4表达水平与病人临床病理特征及预后的相关性

doi:10.3969/j.issn.1005-6483.2023.10.014

摘要/Abstract

摘要： 目的分析结直肠癌(CRC)组织中溴结构域蛋白4(BRD4)的表达，探讨其与病人临床病理特征及预后的关系。方法选取2014年11月~2017年5月本院收治的CRC病人，从病人根治切除术中切取CRC组织及癌旁组织。采用实时荧光定量PCR(qRT-PCR)法、免疫组化法分别检测CRC组织及癌旁组织中BRD4 mRNA及BRD4蛋白表达情况；采用Kaplan-Meier生存曲线分析BRD4表达与病人预后的关系；采用Cox回归分析影响CRC病人预后的危险因素。结果与癌旁组织相比，CRC组织BRD4 mRNA表达水平及BRD4蛋白阳性表达率均升高(P＜0.05)。BRD4 mRNA表达水平与CRC病人区域淋巴结转移(N)、远处转移(M)相关(P＜0.05)，BRD4蛋白表达与CRC病人组织分化程度、N、M相关(P＜0.05)。Kaplan-Meier法分析显示，BRD4 mRNA高表达、BRD4蛋白阳性表达病人5年无进展生存率分别显著低于BRD4 mRNA低表达(P＜0.05)、BRD4蛋白阴性表达(P＜0.05)病人；BRD4 mRNA高表达、BRD4蛋白阳性表达病人5年累积生存率分别显著低于BRD4 mRNA低表达(P＜0.05)、BRD4蛋白阴性表达(P＜0.05)病人。多因素Cox回归分析表明，N₁～N₂、M₁、BRD4 mRNA高表达、BRD4阳性表达是影响CRC病人预后的危险因素(P＜0.05)。结论 CRC组织中BRD4高表达，与病人N、M及预后具有一定的相关性，可作为CRC病人预后的判定指标之一。

关键词: 结直肠癌, 溴结构域蛋白4, 临床病理特征, 预后

Abstract: Objective To analyze the expression of bromodomaincontaining protein 4 (BRD4) in colorectal cancer (CRC) tissue,and to explore its relationship with clinicopathological characteristics and prognosis of patients. Methods CRC patients admitted to our hospital from November 2014 to May 2017 were collected,and CRC tissue and paracancerous tissue were excised from the patients during radical resection.Real-time fluorescence quantitative PCR (qRT-PCR) and immunohistochemistry were used to measure the expression of BRD4 mRNA and BRD4 protein in CRC tissue and paracancerous tissue,respectively;Kaplan-Meier survival curve was used to analyze the relationship between BRD4 expression and patient prognosis;COX regression was applied to analyze the risk factors affecting the prognosis of CRC patients. Results Compared with the adjacent tissues,the expression level of BRD4 mRNA and the positive expression rate of BRD4 protein in CRC tissues were obviously increased (P<0.05).The expression level of BRD4 mRNA was correlated with regional lymph node metastasis (N) and distant metastasis (M) in CRC patients (P<0.05),the expression of BRD4 protein was correlated with the degree of tissue differentiation,N and M in CRC patients (P<0.05).Kaplan-Meier analysis showed that the 5-year progression-free survival rates of patients with high BRD4 mRNA expression and BRD4 protein positive expression were obviously lower than those of patients with low BRD4 mRNA expression (P＜0.05) and BRD4 protein negative expression (P＜0.05).The 5-year cumulative survival rates of patients with high expression of BRD4 mRNA and positive expression of BRD4 protein were significantly lower than those with low expression of BRD4 mRNA (P＜0.05) and negative expression of BRD4 protein (24/32) (P＜0.05) patients.Multivariate COX regression analysis showed that N₁-N₂,M₁,the high expression of BRD4 mRNA and the positive expression of BRD4 were risk factors affecting the prognosis of CRC patients (P<0.05). Conclusion BRD4 is highly expressed in CRC tissue,there is a certain correlation with N,M and prognosis of patients,and it can be used as one of the indicators for the prognosis of CRC patients.

Key words: colorectal cancer, bromodomain-containing protein 4, clinicopathological features, prognosis

达布西力特郑皓韩承新. 结直肠癌组织中溴结构域蛋白4表达水平与病人临床病理特征及预后的相关性[J]. 临床外科杂志, 2023, 31(10): 949-953.

Dabusilite, ZHENG Hao, HAN Chengxin. Correlation of BRD4 expression level in colorectal cancer tissue with clinicopathological characteristics and prognosis of patients[J]. JOURNAL OF CLINICAL SURGERY, 2023, 31(10): 949-953.

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