关键词: 微小RNA-3619-5p, 原发性肝细胞癌, 长链非编码RNA LINC00857, 诊断价值

Abstract: Objective To investigate the expression levels of microRNA-3619-5p(miR-3619-5p) and long noncoding RNA(LncRNA) LINC00857 in serum of hepatocellular carcinoma(HCC) and their clinical diagnostic value.Methods 78 patients with HCC,76 patients with benign liver lesions,and 78 patients with chronic liver disease were taken as HCC group,benign lesion group,and chronic liver disease group,respectively,another 78 healthy subjects who underwent physical examination were taken as the control group.The serum levels of miR-3619-5p,LncRNA LINC00857 and alpha-fetoprotein(AFP) in each group were compared;the diagnostic value of serum miR-3619-5p,LncRNA LINC00857 and AFP for HCC was analyzed.Results Comparison of serum miR-3619-5p [(1.05±0.35),(0.74±0.25),(0.72±0.24),(0.46±0.15)] and LncRNA LINC00,857 [(1.01±0.34),(1.62±0.54),(1.66±0.55),(2.95±0.98)],AFP [(3.13±1.14)μg/L,(48.86±16.29)μg/L,(50.23±18.74)μg/L,(301.25±117.23)μg/L] levels in patients with control group,chronic liver disease group,benign disease group,and HCC group,the difference was statistically significant(P<0.05).The areas under the curve(AUC) of serum miR-3619-5p,LncRNA LINC00857,and AFP for the diagnosis of HCC were 0.870,0.868,and 0.701,respectively,the AUC of serum miR-3619-5p combined with LncRNA LINC00857 to diagnose HCC was 0.956,the sensitivity was 94.9%,and the specificity was 86.4%.Conclusion The expression level of serum miR-3619-5p in HCC patients is low,while the expression level of LncRNA LINC00857 is high.The combination of miR-3619-5p and LncRNA LINC00857 may be more helpful for clinical diagnosis of HCC.

Key words: microRNA-3619-5p, hepatocellular carcinoma, long non-coding RNA LINC00857, diagnostic value