关键词: 膀胱癌, miR-144, 肺癌转移相关转录本1, 睾丸蛋白聚糖, 分化程度, 病理特征

Abstract: Objective To analyze the expression of miR-144,lung cancer metastasis-associated transcript 1 (MALA T1) and testis proteoglycan (SPOCK1) in bladder cancer tissues and their diagnostic efficacy.Methods A total of 96 bladder cancer patients who were treated in our hospital from January 2018 to January 2021 were selected.Fresh tumor tissue from bladder cancer patients (tumor group) and normal tissue adjacent to the tumor > 2 cm away from the tumor edge (paracancerous group) were collected during surgery.5 mg each.The relative expression levels of miR-144,MALAT1 and SPOCK1 in different tissues were detected by polymerase chain reaction (PCR),and the expressions of miR-144,MALAT1 and SPOCK1 in different pathological features of bladder cancer were analyzed.The ROC curve was drawn to test the diagnostic effect of miR-144,MALAT1 and SPOCK1 on bladder cancer.96 patients underwent surgery + postoperative adjuvant chemotherapy in our hospital,and the patients were followed up by telephone and outpatient followup.Followup ended on January 30,2022,and the expressions of miR-144,MALAT1 and SPOCK1 were compared in patients with different prognosis.Results The relative expressions of MALAT1 and SPOCK1 in the tumor group were significantly higher than those in the paracancerous group,while the relative expression of miR-144 was significantly lower than that in the control group (P<0.05).The expression of miR-144 in stage Ⅲ-Ⅳ patients with lymph node metastasis was significantly lower than that in stage Ⅰ-Ⅱ patients without lymph node metastasis（P＜0.05）.MALAT1 and SPOCK levels in stage Ⅲ-Ⅳ,poorly differentiated,and lymph node metastasis were significantly higher than those in stage Ⅰ-Ⅱ,moderately and well differentiated,and no lymph node metastasis (P<0.05).The ROC curve was drawn,and the AUC value was calculated.The AUC values of miR-144,MALAT1 and SPOCK1 for the diagnosis of bladder cancer were 0.643 and 0.557,respectively,and the AUC value for the diagnosis of SPOCK1 was 0.717 (95%Cl:0.688-0.845).the highest AUC value was diagnosed with SPOCK1 (P＜0.05).Follow-up data showed that 85 of 96 patients had good prognosis and 11 had poor prognosis.The miR-144 in the poor prognosis group was significantly lower than that in the good prognosis group,and MALAT1 and SPOCK1 were significantly higher than those in the good prognosis group (P<0.05).Conclusion The abnormal expression of miR-144,MALAT1 and SPOCK1 in bladder cancer tissue has a certain relationship with the pathological characteristics of patients,and may be used as potential diagnostic markers for bladder cancer.

Key words: bladder cancer, MiR-144, lung cancer metastasis-associated transcript 1, testis proteoglycan, degree of differentiation, pathological features